Fig. 3
From: The pathogenic mechanism of syndactyly type V identified in a Hoxd13Q50R knock-in mice
Gene expression analysis in BMP signaling. a The expression levels of Bmp2 during various developmental stages of limb buds. The experimental groups include the mice of wild-type (WT), heterozygotes (Q50R/WT), and homozygotes (Q50R/Q50R). The forelimbs and hindlimbs are represented by “F” and “H,” respectively. “TS” denotes the time with somite numbers. The signals of Bmp2 expression were detected by WISH indicated by red arrows. b Western blot analysis using SMAD1/5 and Phospho-SMAD1/5 in forelimbs (F) and hindlimbs (H) of WT/WT, Q50R/WT and Q50R/Q50R mice at E11.5. c TUNEL staining in interdigital regions of forelimbs from WT/WT and Q50R/Q50R mice at E13.5(TS = 22). Apoptotic cells were red. DAPI was used as a counterstain
