Fig. 4
From: The pathogenic mechanism of syndactyly type V identified in a Hoxd13Q50R knock-in mice
Gene expression analyses of SHH/GREM1/AER-FGF e-m signaling in limb buds by WISH. a The illustration of apical ectodermal ridge (AER) and zone of polarizing activity (ZPA) with A-P axis, P-D axis and D-V axis and the SHH/GREM1/AER-FGF e-m signaling feedback loop. b Grem1 expression pattern in WT/WT, Q50R/WT, and Q50R/Q50R at E10.5 (green boxes marked Grem1 signal). c Shh expression pattern in WT/WT, Q50R/WT, and Q50R/Q50R at E10.5 (red arrows marked Shh signal). d Shh-Fgf8 expression pattern in WT/WT, Q50R/WT, and Q50R/Q50R at E11.5 (red arrows marked Shh signal; blue arrows indicated Fgf8 signal). e Fgf8 expression pattern in WT/WT, Q50R/WT, and Q50R/Q50R at E11.5 (black arrows marked Fgf8 signal; blue double arrows were used as scale bars to indicate that the hindlimbs of WT/WT, Q50R/WT, and Q50R/Q50R were of the same size)
