Fig. 7
Deletion of UTX in ECs reduced senescence in SCMECs and enhanced neurological recovery by downregulating CNN1. a Western blot AND quantification of CNN1 expression in UTXflox/flox, UTX−/− + AAV-NC and UTX−/− + AAV-CNN1 groups after SCI. b Representative images of CD31 (Green) and CNN1 (Red) staining in UTXflox/flox, UTX−/− + AAV-NC and UTX−/− + AAV-CNN1 groups after SCI. Scale bar: 50 μm. c Quantification of relative fluorescence intensity of CNN1 in (b) (n = 4). d Representative SA-β-gal images in UTXflox/flox, UTX−/− + AAV-NC and UTX−/− + AAV-CNN1 groups after SCI. Scale bar: 500 μm. R represents rostral, C represents caudal. e Quantification of relative SA-β-gal intensity in (d) (n = 4). f Elisa test for TNF-α expression in UTXflox/flox, UTX−/− + AAV-NC and UTX−/− + AAV-CNN1 groups after SCI (n = 3). g Elisa test for PAI-1 expression in UTXflox/flox, UTX−/− + AAV-NC and UTX−/− + AAV-CNN1 groups after SCI (n = 3). h Elisa test for CCL-5 expression in UTXflox/flox, UTX−/− + AAV-NC and UTX−/− + AAV-CNN1 groups after SCI (n = 3). i Representative images of CD31 (green) and p21 (red) staining 14days post SCI in UTXflox/flox, UTX−/− + AAV-NC and UTX−/− + AAV-CNN1 groups. Scale bar: 50 μm. The orange arrow indicates CD31+P21+ cell. j Quantification of percentages of CD31+P21+/CD31+ cells in (i) (n = 4). k BMS scores at different timepoints in UTXflox/flox, UTX−/− + AAV-NC and UTX−/− + AAV-CNN1 groups (n = 5). l Neuroelectrophysiology analysis of UTXflox/flox, UTX−/− + AAV-NC and UTX−/− + AAV-CNN1 mice 28 days after SCI. m Quantification of motor evoked potential and latent period in (l) (n = 5). n Representative images of Tuj-1 staining of UTXflox/flox, UTX−/− + AAV-NC and UTX−/− + AAV-CNN1 mice 28 days after SCI. Scale bar: 500 μm. Scale bar for magnified images: 50 μm. The asterisk indicates the lesion center. o Quantification of relative Tuj-1 densities in (n) (n = 4). Data are presented as the mean ± SD. **P < 0.01, #P < 0.05, ns=not significant
