Fig. 8

UTX knockdown depressed cell senescence and improved endothelial activity by downregulating CNN1 in vitro. a Western blot analysis of CNN1, Lamin B1, P16, P21 and P53 expression in UTX-NC, UTX-KD and UTX-KD + CNN1-OE groups. b Quantification of CNN1 expression in (a) (n = 3). c Quantification of LaminB1 expression in (a) (n = 3). d Quantification of P53 expression in (a) (n = 3). e Quantification of P21 expression in (a) (n = 3). f Quantification of p16 expression in (a) (n = 3). g Representative images of SA-β-gal staining in UTX-NC, UTX-KD and UTX-KD + CNN1-OE groups. Scale bar: 100 μm. h Quantification of SA-β-gal⁺ cells in (g) (n = 4). i Representative images of CD31 (green) and P53 (red), CD31 (green) and P21 (red) in UTX-NC, UTX-KD and UTX-KD + CNN1-OE groups. Scale bar: 20 μm. j Quantification of percentages of CD31⁺P53⁺/CD31⁺ cells in (i) (n = 4). k Quantification of percentages of CD31⁺P21⁺/CD31⁺ cells in (i) (n = 4). l Representative images of bEnd.3 canaliculization (upper panel) and transwell migration (lower panel) in UTX-NC, UTX-KD and UTX-KD + CNN1-OE groups. Scale bar: 100 μm. m Quantification of segments lengths in (l) (n = 4). n Quantification of migrated cells in (l) (n = 4). o Representative images of the horizontal migration in UTX-NC, UTX-KD and UTX-KD + CNN1-OE groups. Scale bar: 200 μm. p Quantification of relative migration rate in (m) (n = 4). Data are presented as the mean ± SD. **P < 0.01