Fig. 4 | Bone Research

Fig. 4

From: Lumbar instability remodels cartilage endplate to induce intervertebral disc degeneration by recruiting osteoclasts via Hippo-CCL3 signaling

Fig. 4

Abnormal stress regulates the Hippo signaling in CEPCs. a KEGG analysis of the CEPCs before and after 12% CTS stimulation. b Gene set enrichment analysis of the differentially expressed genes in 12% CTS versus control. c Left, IHC of YAP expression in CEP of mice with LSI or sham surgery for 8 weeks. Right, statistical analysis of the percentage of YAP positive chondrocytes. d, e Top, IF staining of YAP and p-YAP in CEPCs with Ctrl, 5% CTS and 12% CTS. Bottom, fluorescence colocalization analysis of YAP and DAPI following the white line shown in staining. F-actin (red), YAP and p-YAP (green), DAPI (blue). f, g Quantitative analysis of the mean fluorescence intensity of CESCs in (d) and (e), respectively. h WB analysis of YAP and p-YAP expression in CEPCs treated with CTS. *, P < 0.05, **, P < 0.01

Back to article page