Fig. 9 | Bone Research

Fig. 9

From: Lumbar instability remodels cartilage endplate to induce intervertebral disc degeneration by recruiting osteoclasts via Hippo-CCL3 signaling

Fig. 9

Yap1-AAV5 protected CEP from remodeling in LSI mice. a LSI or sham mice CEP treated with paravertebral injection of Yap1-AAV5. The L4-5 level of CEP were harvested at 8 weeks after injection of Yap1-AAV5 in LSI or sham mice. b Tracing of Yap1-AAV5 in the CEP of L4/5 by IF staining of Yap1-AAV5 (Green), YAP (Red) and DAPI (blue). c Representative 3D Micro-CT images of the mice caudal endplates of L4-5 level (coronal view) at 8 weeks after LSI or sham surgery. d, e Quantitative analysis of the total porosity and Tb. Sp based on (c). f Representative SOFG staining of the CEP from LSI or sham mice injected with or without Yap1-AAV5. g Endplate score of the caudal endplates based on (f). h, i, j IHC of Collagen II, Collagen X and TNF-αin CEP from LSI or sham mice injected with or without Yap1-AAV5

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