Fig. 3

HOXC10 inhibition attenuates osteolytic bone metastasis. a, b TRAP staining for primary preosteoclasts induced by H441 and A549 cells CM (a). The TRAP-positive multinucleated osteoclasts were analyzed (b). c qPCR validation of MMP9 mRNA levels in H441 and A549 cells. d Representative IVIS bioluminescence imaging of H441 cells injected into BALB/c nude mice (6 weeks) via intracardiac injection at day 42 (n = 6 per group). e The growth of bone metastasis in (d) was quantified. f Representative micro-CT images of spine from the moribund intracardiac injection mice. Red arrows, osteolytic lesions. Scale bars, 1 mm. g Bar graph of quantitative osteolytic lesions of spine from the moribund mice (n = 3 per group). h Representative TRAP staining in bone of mice (n = 3 per group). i Survival curves for indicated mice (n = 6 per group). Scale, 50 μm. j Scheme of the mini-PDX models. k The relative cell viability shows a significant decrease in AAV-shHOXC10 mice post AAV treatment (n = 6 per group). Scale bars, 100 μm. Data in (b) represent three technical replicates, representative of three independent experiments with similar results. Data in (b, c, e, g, k) shown as mean ± s.e.m., (b) was performed one-way ANOVA with Tukey’s multiple comparison test, c, g, k performed unpaired two-sided Student’s t test, (h) performed log-rank test, *P < 0.05, **P < 0.01, ***P < 0.001