Fig. 1 | Bone Research

Fig. 1

From: Hydroxychloroquine and a low antiresorptive activity bisphosphonate conjugate prevent and reverse ovariectomy-induced bone loss in mice through dual antiresorptive and anabolic effects

Fig. 1

HABP-HCQ stimulates osteoblast and inhibits osteoclast differentiation. a Synthesis of HABP – Hydrolysis of tetraethyl HABP with bromotrimethylsilane. b C57BL/6 mouse BM cells were cultured with M-CSF for 2 d followed by treatment with RANKL plus the indicated doses of HCQ, HABP-HCQ, or HABP for 3 d. OC number and surface were evaluated after TRAP staining. c BM cells were cultured for 5 d in 24-well plates to expand stromal cells, which were induced for OB differentiation in the presence of the indicated doses of HCQ or HABP-HCQ intermittently, 6 h each day, for 5 d. ALP staining was performed to evaluate OB differentiation. d Effects of 1 μmol/L HABP vs. 1 μmol/L HABP-HCQ and HCQ on OB differentiation, tested in 12-well plates, as above in (b). *P < 0.05 & **P < 0.01 vs. vehicle, or #P < 0.01; one-way ANOVA+Dunnett’s test

Back to article page