Fig. 2 | Bone Research

Fig. 2

From: Hydroxychloroquine and a low antiresorptive activity bisphosphonate conjugate prevent and reverse ovariectomy-induced bone loss in mice through dual antiresorptive and anabolic effects

Fig. 2

HABP-HCQ stabilizes TRAF3 in osteoclast and osteoblast progenitor cells. a C57BL/6 mouse BM cells were cultured with M-CSF for 3 d to expand macrophages, which were treated with vehicle or RANKL plus 3 μmol/L of the indicated compounds for 24 h. Protein levels of TRAF3 and its related level normalized to β-actin were analyzed by WB. b BdMPCs were treated with TGFβ1 plus 3 μmol/L of the indicated compounds for 24 h, and levels of TRAF3 and β-actin protein were analyzed by WB. *P < 0.05 & **P < 0.01 vs. vehicle, #P < 0.01 between the two groups, one-way ANOVA + /Dunnett’s test

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