Fig. 3 | Bone Research

Fig. 3

From: Hydroxychloroquine and a low antiresorptive activity bisphosphonate conjugate prevent and reverse ovariectomy-induced bone loss in mice through dual antiresorptive and anabolic effects

Fig. 3

Low dose of HABP-HCQ effectively prevents continuous PTH-induced osteoclast formation and bone marrow fibrosis in mice. a Diagram showing the treatment schedule of HABP-HCQ vs HCQ and PTH in mice. b TRAP-stained calvarial bones were used to quantify OC number (upper panel, black * = calvarial bone; red arrows = OCs formed in the suture) and H&E-stained tibial bones were used to quantify fibrosis around trabeculae (lower panel, black * = trabecular bone; yellow arrows = fibroblastic cells around the trabeculae; black arrows = osteoblasts on trabecular surfaces). c An independent experiment was performed to evaluate the effect of HABP on PTH-induced OC formation in calvarial bones (upper panel, black * = calvarial bone, red arrows = OCs formed in the suture) and BM fibrosis in tibial bones (lower panel, black * = trabecular bone, yellow arrows = fibroblastic cells around the trabeculae). 5 mice per group for all experiments. *P < 0.05, **P < 0.01 vs. Vehicle or the two groups with line, one-way ANOVA+Dunnett’s test

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