Fig. 5
Low-dose HABP-HCQ restores the lost bone caused by OVX by inhibiting bone resorption and maintaining high levels of bone formation. 10-week-old female C57BL/6 mice had sham (1 group) or OVX surgery (8 groups; 8-9 mice per group. 4 weeks post-surgery, the sham and one group of ovariectomized mice were micro-CT scanned alive to confirm bone loss in ovariectomized mice (which then were euthanized). From the 5th week, the 8 groups of ovariectomized mice were randomly assigned to 5 doses per week of the indicated treatments for 5 weeks. a The spines were micro-CT scanned to quantify the bone structural parameters in L1 vertebrae. b TRAP-stained sections from L4 vertebrae were used to quantify OC numbers and surfaces on eroded surfaces. c OB surfaces on trabeculae were quantified on H&E-stained sections of L4 vertebrae. d Dynamic bone formation parameters were measured in plastic-embedded undecalcified sections from L1 vertebrae. *P < 0.05, **P < 0.01, one-way ANOVA+Dunnett’s test
