Fig. 2
From: Targeting Fascin1 maintains chondrocytes phenotype and attenuates osteoarthritis development
Targeted deletion of FSCN1 in chondrocytes prevents OA development in mice. a Twelve-week-old FSCN1flox/flox (FSCN1-WT) and FSCN1-iKO mice underwent DMM or sham surgery 1 week after tamoxifen injection. The knees were harvested at 6 or 10 weeks postoperatively for histological analysis (n = 8). b IF staining of FSCN1 in articular cartilage from FSCN1-WT and FSCN1-iKO mice at 6-weeks post-surgery. c SOFG staining of joints from FSCN1-WT and FSCN1-iKO mice in sham group displayed with growth plate. d, e SOFG staining of joints from FSCN1-WT and FSCN1-iKO mice with sham or DMM-induced OA at 6- or 10-weeks post-surgery. The inset in each image is shown as a magnified image in the bottom row. f Cartilage destruction (OARSI grades) and synovial inflammation were determined by SOFG staining and scored (n = 8). g IHC staining of MMP3 and MMP13 in articular cartilage from FSCN1-WT and FSCN1-iKO mice at 6 or 10 weeks post-surgery. h IF staining of Collagen type II (green) and III (red) in articular cartilage from FSCN1-WT and FSCN1-iKO mice at 6- or 10-weeks post-surgery. Quantified results of each set of data are shown below (n = 8). Scale bars, 50 μm. All data are presented as means ± SEM
