Fig. 6
From: Targeting Fascin1 maintains chondrocytes phenotype and attenuates osteoarthritis development
FSCN1 inhibitor NP-G2-044 ameliorates OA progression at early and middle stages in mice. a Twelve-week-old C57BL6/J mice underwent DMM or sham surgery, and saline (control), or NP-G2-044 (10 mmol/L) was injected into the knee joints every week after surgery. The knees were harvested at 6 or 10 weeks postoperatively for histological analysis (n = 8). b SOFG staining of joints from mice with DMM-induced OA and treated with control or with NP-G2-044 at 6 or 10 weeks post-surgery. The insets in the images are shown as magnified images in the bottom row. c cartilage destruction (OARSI grades) and synovial inflammation were determined by SOFG staining and scored (n = 8). IHC staining of MMP3 and MMP13 (d), IF staining of Collagen type II (green) and III (red) (e) and p-Smad1/5 (green) and β-catenin (red) (f) in articular cartilage from control mice and those treated with NP-G2-044 at 6 or 10 weeks post-surgery (n = 8). Scale bars, 50 μm. All data are presented as means ± SEM
