Fig. 4

Cellular Interaction Analysis. a Schematic detailing how the Delaunay Triangulation method is used to establish cell-to-cell contacts between cellular populations of the iTME. Schematic adapted from the Enable Medicine Inc. User Manual and previous publication.¹⁶⁰ b Volcano plot depicting cellular interactions within metastatic OS specimens, stratified by 5-year survival status. Red line indicates P < 0.05, with any cell type above considered statistically significant. c Representative interaction chord diagram of a 5-year deceased metastatic OS core (left) with enrichment for PD-1⁺ Memory Helper T cell interactions (right). d Representative interaction chord diagram of a 5-year deceased metastatic OS core (left) with enrichment for MDSC interactions (right). e Representative interaction chord diagram of a 5-year survivor metastatic OS core (left) with enrichment for PDPN⁺ CD21⁺ B cell interactions (right). The 5-year deceased cohort represents n = 8 patients, 18 cores while the 5-year survivor represents n = 4 patients, 8 cores. Welch’s t-test was conducted to assess the differences in means between the compared cohorts, with significance set at P < 0.05 (Enable Medicine Cloud Platform)