Fig. 5

Cellular Neighborhood Analysis. a Cellular neighborhood (CN) identification in all metastatic OS specimens using k-means clustering, 10 neighbors, and 12 CN classifications. Rows represent the CNs while columns represent cell type. Heatmap is scaled by rows (0-1, giving cell type proportions within each neighborhood) and then by columns (global abundance of cell types) followed by a log₂ transformation. b Heatmap, normalized to Min-Max, depicting the CN composition of metastatic OS specimens, stratified by 5-year survival status. Each row represents a single core from the TMA. c Volcano plot depicting CN enrichment within metastatic OS specimens, stratified by 5-year survival status. Red line indicates P < 0.05, with any CN above considered statistically significant. d Representative Voronoi plot of a 5-year deceased metastatic OS core. e Representative Voronoi plot of a 5-year survivor metastatic OS core. The 5-year deceased cohort represents n = 8 patients, 18 cores while the 5-year survivor represents n = 4 patients, 8 cores. Welch’s t-test was conducted to assess the differences in means between the compared cohorts, with significance set at P < 0.05 (Enable Medicine Cloud Platform)