Fig. 6

OS Spatial Score. a Schematic diagram depicting the calculation of the OS Spatial Score by dividing the distance of Tregs, TAMs, and MDSCs to Natural Killer (NK) cells (left) by the distance of Tumor cells to NK cells (right). Increased effector function correlates with an increased Spatial Score, while decreased effector function correlates with a decreased Spatial Score. Schematic created at BioRender.com. b Treg-, TAM-, and MDSC-NK-Tumor Spatial Scores for metastatic OS specimens, stratified by 5-year outcome. Each dot on the box plot denotes a single core from the TMA. c Composite OS Spatial Score depicts increased effector activity in the 5-year survivor cohort. Each dot on the box plot denotes a single core from the TMA, with whiskers extending from the Min to Max value. The 5-year deceased cohort represents n = 8 patients, 18 cores while the 5-year survivor represents n = 4 patients, 8 cores. Welch’s t-test was conducted to assess the differences in means between the compared cohorts, with significance set at P < 0.05 (GraphPad Prism 10)