Fig. 1
From: Bone loss with aging is independent of gut microbiome in mice
The workflow of study design. a 1-month-old CB6F1 germ-free (GF) mice were randomly assigned to 2 experimental groups: remain GF or colonization with microbiome from 3-month-old donors for 23 months. b Fecal samples were collected from 3-month-old and 24-month-old conventionally raised specific pathogen free (SPF) mice for gut microbiota characterization by 16S rRNA gene sequencing and LC-MS. 2-month-old CB6F1 GF mice were randomly assigned to remain GF or be colonized with fecal microbiome from 3-month-old or 24-month-old donors for (c) 1 month or (e) 8 months. d 1-month-old CB6F1 GF mice were randomized to remain GF or be colonized with microbiome from 3-month-old or 24-month-old donors for 1 month
