Fig. 3
From: Bone loss with aging is independent of gut microbiome in mice
Distinct microbial compositions between young and old male mice. Principal coordinate analysis (PCoA) plots of (a) weighted UniFrac distance and (b) Bray Curtis distance were used to visualize microbial β-diversities. Significance was determined by permutational multivariate analysis of variance (PERMANOVA) with 999 permutations, (a, b) showing a significant microbial community shift between the young (red dots) and old (blue dots) mice. c α-diversity was measured by Simpson index. d Mean relative abundance of taxa at phylum level. e Firmicutes to Bacteroidetes (F/B) ratio was calculated. f Cladogram of Linear discriminant Effect Size (LEfSe) analysis. g At the genus level, 6 discriminant taxa were identified by LEfSe analysis. Mann Whitney U test was performed for (c–e). For (d), *P < 0.05, **P < 0.01. 3-month, n = 8; 24-month, n = 8
