Fig. 4
From: Bone loss with aging is independent of gut microbiome in mice
PICRUSt2 predictions of microbial metagenome functions based on 16S rRNA gene. Metagenomic function was predicted using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Young and old mice demonstrated different microbial predicted metagenomic functions in (a) energy and fermentation, (b) carbohydrate degradation, (c) vitamin biosynthesis, (d) fatty acid and lipid biosynthesis, and (e) protein and amino acid biosynthesis. Data are TMM (trimmed mean of M values) normalized and represented as mean with 95% confidence intervals. Unpaired t-test or Mann Whitney U test was performed. *P < 0.05, **P < 0.01, ***P < 0.001. 3-month, n = 8; 24-month, n = 8
