Fig. 6
From: Bone loss with aging is independent of gut microbiome in mice
Integrative analysis of 16S microbiome and LC-MS metabolome. Integrative analysis of 16S microbiome and LC-MS metabolome was performed using a customized gene set enrichment analysis (GSEA) method. Metabolites obtained by LC-MS were significantly enriched in (a) amino acid biosynthesis and (b) aminoacyl-tRNA charging using the predicted metagenome of 16S rRNA gene dataset (P < 0.05). Peak intensity of metabolites involved in (c) amino acid biosynthesis and (d) aminoacyl-tRNA charging was log transformed and visualized by heatmap, with each row representing a unique sample and each column representing a LC-MS peak. For (c, d), *P < 0.05, **P < 0.01, ***P < 0.001. For LC-MS, 3-month, n = 5; 24-month, n = 5. For 16S, 3-month, n = 8; 24-month, n = 8
