Fig. 6
From: Photothermal sensitive nanocomposite hydrogel for infectious bone defects
Evaluation of cytotoxicity and osteogenesis of bone marrow stem cells (BMSCs). a Identification of BMSCs: surface markers of BMSCs were detected by flow cytometry. Cells exhibiting CD44+, CD73+, CD34-, and CD45- were identified as BMSCs. b Double staining of live/dead cells. The green regions represent live cells, while the red regions represent dead cells. (scale bar = 250 μm). c The survival rate of BMSCs was assessed on days 1, 3, and 5 using the CCK-8 assay. d Quantitative analysis of ALP activity was quantified on days 3 and 7. *P < 0.05. Two-way ANOVA (n = 3). e Quantitative analysis of Alizarin Red Staining (ARS) staining was performed on days 14 and 21. *P < 0.05. Two-way ANOVA (n = 3). f Representative images of ALP staining performed on day 7 (scale bar = 500 μm). g ARS staining on day 21 is presented (scale bar = 250 μm)
