Fig. 3
Deletion of FXR in osteoclasts did not affect delayed bone development and osteoporosis phenotype. a Illustration of osteoclast lineage-specific FXR knockout mice, which were generated via the crossbreeding of FXRfl/fl mice with Ctsk-Cre transgenic mice. b Western blot analysis of FXR in BMMs from 4-week-old male FXRfl/fl and FXRctsk mice cultured with M-CSF and RANKL for 7 days. c Representative double-stained with alcian blue and alizarin red S image of skeletal preparations from FXRfl/fl and FXRCtsk mice newborns. Scale bar = 5 mm. d–g Representative micro-CT images of distal femurs in 8-week-old FXRfl/fl and FXRCtsk mice with morphometric analysis of bone volume per tissue volume (BV/TV), trabecular number (Tb.N), trabecular spacing (Tb.Sp). n = 6. h Representative TRAP staining images of BMM from FXRfl/fl and FXRCtsk mice. Scale bar = 50 μm. i Heatmap of FXR and osteoclast-related genes mRNA expression in osteoclastic differentiation (n = 6). ns no significant difference. Data are represented as mean ± SEM
