Fig. 7

Conditional SIRT6 ablation in SCs impairs GMT and subsequent bone repair. a Experimental scheme for conditional SIRT6 deletion in SCs from jaw bone healing mice model. b Representative images of μCT reconstruction of the alveolar bone regeneration at day 7 post tooth extraction in Sirt6^fl/fl and Plp1-Sirt6^fl/fl mice. The quantitative analysis of BMD and BV/TV was shown (n = 6). c H&E staining of TES at day 7 and day 14 post tooth extraction (n = 6). Scale bar: 100 μm. d Dynamic histomorphometry of trabecular bone (Tb) with quantification of MAR in TES (n = 6). Scale bar: 5 μm. e Representative images of Shh immunostaining in healing sockets at day 7 post tooth extraction and the relative quantification (n = 6). Scale bar: 100 μm. f Representative images of tdTomato⁺ cells and Gli1 immunostaining in TES at day 7 post tooth extraction and relative quantification per socket (n = 6). Scale bar: 100 μm. g Representative images of CD31 and EMCN immunostaining and quantification of CD31⁺EMCN⁺ type H vessels in TES (n = 6). Scale bar: 100 μm. h Reporter vectors (Shh E1 and E3-site-HSVtk promoter-GFP) were transfected into tooth extraction sockets by local delivery. Representative images of tdTomato⁺ SCs and GFP expression were shown and the percentage of tdTomato⁺GFP⁺ cells per socket was analyzed (n = 3). Scale bar: 100 μm. Data were presented as mean ± SD; **P < 0.01, ***P < 0.001