Fig. 7 | Bone Research

Fig. 7

From: Enhanced SIRT3 expression restores mitochondrial quality control mechanism to reverse osteogenic impairment in type 2 diabetes mellitus

Fig. 7

Sirt3-overexpression rescues HFD&STZ-induced mitophagy defects. a Schematic illustration of the establishment T2DM with injection of Adeno-associated virus (AAV) overexpressing Sirt3. b IHC staining for SIRT3 of bone sections in CTRL and Sirt3OE group from normal and HFD&STZ mice. Scale bar, 100 μm. c Representative IF images of bone sections showing co-localization of OCN (red), SIRT3 (deep red), and AAV-GFP (green). Scale bar, 100 μm. d Representative images and quantification of mitochondria stained with MitoTracker Green of primary osteoblasts from above groups. e The presentative IF images of PINK1 and PRKN with mitochondria in primary osteoblast from CTRL, Sirt3OE, HFD&STZ, and HFD&STZ+Sirt3OE mice. f The presentative IF images of PINK1 and PRKN in bone tissues. g, h Western blot images and quantification of mitophagy markers in bones from each group. Data presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

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