Fig. 8 | Bone Research

Fig. 8

From: Enhanced SIRT3 expression restores mitochondrial quality control mechanism to reverse osteogenic impairment in type 2 diabetes mellitus

Fig. 8

Overexpression of Sirt3 reverses bone loss in HFD&STZ mice. a Representative micro-CT images of femurs from different groups of mice. b Quantification of bone parameters by means of micro-CT, including BV/TV, Tb.Sp, Tb.N, and BS/TV. c Representative images of H&E staining of bone sections from different groups. d, e High magnification and quantification of xylenol orange double labeling of the femur from different group mice. Scale bar, 50 μm. f, g IHC staining against OCN and RUNX2 of bone sections. Scale bar, 100 μm. h Serum concentration of PINP. n = 6 per group. i qPCR analysis of osteogenesis genes including Bglap, Runx2, Col1a1 mRNA levels. n = 6 per group. j Western blot images and quantification including ALP, RUNX2, and COL1A1 proteins of bone tissue from different group. k, l Representative images and quantification of ALP (7 days) and ARS (21 days) staining of primary osteoblasts isolated from mice in different group. m Western blot analysis of RUNX2, COL1A1, and OPN proteins in primary osteoblasts from abovementioned groups. Data presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

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