Fig. 6
From: Porcupine inhibition is a promising pharmacological treatment for severe sclerosteosis pathologies
LGK974 treatment results in reduced bone morphometric parameters and bone turnover marker expression in Sost-/- lumbar vertebrae. a Vertebral regions selected for µCT analysis highlighted in white. b Representative 3D µCT images of the L4 vertebral bodies of treated and untreated Sost-/- mice (male and female). Effect of LGK974 treatment on L4 lumbar vertebral body (c) TV, (d) BV, (e) BV/TV, (f) trabecular thickness (Tb.Th), (g) number (Tb.N) and (h) space (Tb.Sp). Relative (i) Col1a, (j) Axin2 and (k) Trap gene expression in Sost-/- lumbar vertebrae. l, m Representative IHC (AXIN2) images of vehicle and LGK974 treated male Sost-/- lumbar vertebrae, with secondary (2y) antibody only control included as inset in (m). Black = AXIN2 positive osteocytes (yellow arrows) and Blue = AXIN2 negative osteocytes (white arrows). n AXIN2 positive (AXIN2+) osteocytes (statistical analysis; Welch’s t-test). For all graphs, means of each group are indicated by the solid line, and upper and lower dashed lines represent quartiles. Blue and red represent vehicle controls and LGK974 treated groups, respectively. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 and ****P ≤ 0.000 1
