Fig. 7
From: Porcupine inhibition is a promising pharmacological treatment for severe sclerosteosis pathologies
LGK974 treatment reduces tibial cortical bone morphometric parameters in Sost-/- mice. a Colour coded (heat map of cortical bone thickness), representative 3D µCT images of whole, loaded tibiae (male and female) treated with vehicle and LGK974. Representative 2D µCT cross sections of loaded tibiae are also shown. b Cortical thickness heat maps of whole, non-loaded tibiae (male and female) treated with vehicle control and LGK974. Representative 2D µCT cross sections of non-loaded tibiae are also shown. c Representative image of a Sost-/- mouse tibia that indicates the analysed region (black rectangle). Effect of LGK974 on cortical (d, e) bone and tissue volume (BV and TV), and (f) bone area (B.Ar) of Sost-/- male non-loaded and loaded tibiae. g–i Effect of LGK974 on the same cortical parameters of female tibiae. Representative images of alizarin red (days 4 and 11) and calcein green (days 18 and 25) labelled tibial cortical bone surfaces of male (j) control and (k) LGK974 treated Sost-/- mice. l Percentage mineral surface (MS/BS) and (m) bone formation rate (BFR/BS) of control and LGK974 treated male loaded tibia (alizarin red dual labelled). (n) Percentage mineral surface (MS/BS) and (o) bone formation rate (BFR/BS) of control and LGK974 treated male loaded tibia (calcein green dual labelled). Line graphs (f, i) represent means ± SEM (1 = non-loaded control (dark blue); 2 = non-loaded LGK974 treated (light blue); 3 = loaded control (maroon); 4 = loaded LGK974 treated (red)). Graphical heat maps below line graphs summarise statistical differences of the above groups 1-4 at specific matched locations along the analysed length of the tibiae and represent the overall effect of LGK974 treatment (dark blue P ≥ 0.05, blue 0.01 ≤ P < 0.05, green 0.001 ≤ P < 0.01, yellow P < 0.001). Interaction is between LGK974 treatment and loading. For violin plots, means of each group are indicated by the solid line, and upper and lower dashed lines represent quartiles. Blue and red represent vehicle controls and LGK974 treated groups, respectively. *P ≤ 0.05, **P ≤ 0.01 and ****P ≤ 0.000 1. Group sizes were n = 5 treated females, n = 6 control females, n = 4 treated males and n = 5 control males. Dynamic histomorphometry group sizes were n = 4 control and n = 4 treated males, with two levels analysed for each tibia
