Fig. 2
From: Apolipoprotein E is a marker of all chondrocytes in the growth plate resting zone
Characterization of RCs by scRNAseq and FISH to identify markers for RCs. a Reclustering and UMAP visualization of the GP chondrocytes selected in Fig.1a. b Gene panel used for cluster annotation of GP chondrocytes and FISH validation. c FISH images and the corresponding feature plots of genes representing chondrocytes in different zones. d Pseudotime analysis of the GP chondrocytes. Higher pseudotime scores indicate more mature/differentiated chondrocytes. e Heatmap of the detected genes that are ubiquitously and specifically expressed in the RC cluster. f FISH for Apoe, C1qtnf3, and Col10a1 on a coronal section of a 4-week-old male mouse tibia. Feature plot of the three genes is shown in corresponding colors next to the FISH image. g Hematoxylin & Eosin (H&E) staining and immunofluorescence staining of human APOE on sections of the human radial growth plate. At least 3 mice were used for each gene and the representative images were shown
