Fig. 3

Systemic and skeletal phenotypes of newly generated Apoe^mCherry/+ mice. a Gene editing strategy to generate Apoe^mCherry knock-in mice. b Body weights (Apoe^+/+ males = 19, Apoe^+/+ females = 11, Apoe^mCherry/+ males = 12, Apoe^mCherry/+ females = 13), femur lengths measured by micro-CT scanning (Apoe^+/+ males = 11, Apoe^+/+ females = 9, Apoe^mCherry/+ males = 12, Apoe^mCherry/+ females = 11), plasma APOE levels measured by ELISA (n = 5 for each group), and blood lipid levels measured by colorimetric assays (n = 5 for each group except the total cholesterol level in the Apoe^mCherry/+ group which is n = 4). Samples were from independent animals. Data were presented as mean ± SD. Two-way ANOVA followed by Tukey’s multiple comparison test. c Tibial sections from 4-week-old male Apoe^+/+ and Apoe^mCherry/+ mice (n = 3 independent animals) were stained by FISH for Apoe, C1qtnf3, and Col10a1 to label the RZ, PZ, and HZ, respectively. Percentage of each zone within the entire growth plate was quantified and compared by Two-way ANOVA followed by Tukey’s multiple comparisons test. Data are presented as mean ± SD. ns: P > 0.05