Fig. 3

CD47 is required for cell colony expansion and proliferation. Whole marrow and periosteum were harvested from the femur and tibia of WT and CD47-null mice. a CFU-F of WT (n = 8) and CD47-null (n = 12) marrow-derived cells. b Representative CFU-f plate from WT (left) and CD47-null (right) mice. c MTT assay of primary marrow-derived MSC from WT (n = 4) and CD47-null (n = 4) mice at 1, 6, 10 and 14 days in culture. d, MTT assay of marrow-derived MSC pooled from two to three mice (equivalent ratios of males and females), WT (n = 6 pools), and CD47-null (n = 6 pools) after the 1st passage at 3, 6 and 9 days in culture. e CCK8 assay of magnetically sorted CD45- MSC from WT (n = 4) and CD47-null (n = 4) after 1st passage at 1, 3, 5, 7 and 9 days. f CCK8 assay of magnetically sorted CD45⁺ immune cells from WT (n = 4) and CD47-null (n = 4) after 1st passage at 1, 3, 5, 7 and 9 days. g CCK8 assay of periosteal MSC from WT (n = 8) and CD47-null (n = 8) after the 1st passage at 1, 3, 5, 7 and 9 days in culture. h CFU-F of periosteal MSC from WT (n = 6) and CD47-null (n = 6) mice. Unless otherwise stated, each data point represents an individual mouse. Mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 two-sided t tests