Fig. 4

Loss of CD47 decreases MSC pluripotency, proliferation, and apoptosis genes and decreased CD47-null MSC are in S/G2 phase. Gene expression analysis of MSC harvested from the femur and tibia of WT (n = 3) and CD47-null (n = 4) after the 1st passage. Each data point represents the pooling of two to three mice. a Cd47 gene expression. Pluripotent stem cell genes, b Oct4, c Klf4, d c-Myc. Pre-osteoblast gene e alkaline phosphatase (ALP), apoptosis associated gene, f caspase 3 (CAP3), and proliferation marker g forkhead box M1 (FOXM1). Graphs indicate WT and CD47-null gene expression normalized to the housekeeping gene, GAPDH, relative to WT (n = 3) mice. Cell cycle analysis of marrow-derived MSC harvested from the femur and tibia of WT (n = 5) and CD47-null (n = 5) following first passage. h Representative histograms of WT (left) and CD47-null (right) with cell cycle analysis overlays. i Percent of cells in G1 phase or S/G2. Mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, two-sided t test