Fig. 6
From: CD47 is required for mesenchymal progenitor proliferation and fracture repair
Genetic knockout of CD47 limits recovery of whole limb perfusion, but shows local increases in endothelial cells after induced ischemia. Relative limb perfusion of WT and CD47-null mice at days 0–9 post-ischemic surgery using laser Doppler flowmetry. a Perfusion data was fit to a one-phase non-linear curve for WT (fit is the solid blue line with 95% CI in transparent blue; R2 = 0.760 9) and CD47-null (fit is the solid red line with 95% CI in transparent red; R2 = 0.622 3). The rate of recovery was faster, but maximum recovery was significantly lower in CD47-null compared to WT mice (P < 0.000 1, extra sum-of-squares F test). b Representative immunofluorescence staining of CD31 and EMCN in CD47-null (n = 8) and WT (n = 7) ischemic fractures at 20× (Scale bar = 100 µm). c CD31 quantification in WT and CD47-null ischemic fracture calluses at day 4 post-fracture at peripheral and central regions of the fracture callus. d EMCN quantification in WT and CD47-null ischemic fracture calluses at day 4 post-fracture at peripheral and central regions. Each data point represents an individual mouse. Mean ± SD, *P < 0.05, **P < 0.01, two-sided t test
