Fig. 2
ZBTB20 icKO in chondrocytes alleviates OA. a Safranin O/Fast Green staining of articular cartilage from Zbtb20-icKO or corresponding control mice underwent DMM or Sham surgery for 8/12 weeks. Panels in the upper right corner are high magnifications of images. The arrows indicated the hypertrophy-like chondrocytes. n = 8, 9, 9, 9, 4, 3, 4, 3 mice. b Statistical analysis of OARSI scores, thickness of hyaline cartilage (HC), calcified cartilage (CC) and ratio of HC to CC in indicated groups of mice in (a). c Representative images of knee joint osteophytes (top panel) and subchondral bone plate (bottom panel) reconstructed by MicroCT analysis from indicated group of mice. n = 4, 3, 4, 3 mice. d Statistical analysis of volume of osteophytes (panel on the left) and SBP thickness (panel on the right) in indicated groups of mice in (c). e IF staining of COL II, ACAN, MMP13, and ADAMTS5 in the tibial cartilage from indicated group of mice. The dashed lines mark the edge of cartilage or the tidemark. The arrows mark the MMP13+ or ADAMTS5+ chondrocytes. f Statistical analysis of IF signal in indicated groups of cells in (e). n = 3, 3, 3, 3 mice. g Western blot of ZBTB20, COL II, MMP13, and ADAMTS5 in IL-1B treated zbtb20f/f primary chondrocytes transduced with Ad-GFP or Ad-Cre-GFP. h Relative mRNA levels of Zbtb20, Col2α1, Mmp3 and Adamts5 in chondrocytes described in (g). i Western blot of ZBTB20, COL II, MMP13, and ADAMTS5 in IL-1B treated primary chondrocytes transfected with Ad-GFP or Ad-mZbtb20-GFP. j Relative mRNA levels of Zbtb20, Col2α1, Mmp3, and Adamts5 in chondrocytes described in (i). g–j n = 3 biological independent experiments
