Fig. 3
Activation of NF-κB signaling by ZBTB20 requires suppression of Pten. a Scatter plot graph showing the expression changes of DEGs from each comparison. b Dot plot graph of the top 10 enriched KEGG pathways of DEGs from each comparison. a, b n = 3 replicates. c Western blot of pAKT-T308, pAKT-S473, and AKT in indicated chondrocytes. d Statistical analysis of the bands in (c). e Representative tracks of CUT&Tag-seq analysis showing the enrichment of ZBTB20 around Pten’s promoter in chondrocytes. f Relative enrichment of ZBTB20 on binding regions compared to IgG in chondrocytes treated by IL-1β or not analyzed by ChIP-qPCR. g Western blot of PTEN, pp65-S536 and p65 in IL-1B treated zbtb20f/f primary chondrocytes transduced with Ad-GFP or Ad-Cre-GFP. h Relative mRNA levels of Zbtb20 and Pten in chondrocytes described in (g). i Western blot of PTEN, pp65-S536 and p65 IL-1B treated primary chondrocytes transduced with Ad-GFP or Ad-mZbtb20-GFP. j Relative mRNA levels of Zbtb20 and Pten in chondrocytes described in (i). k, l Relative mRNA levels of Zbtb20, Mmp3, and Col2α1 in indicated groups of chondrocytes treated with IL-1β or/and SC79/ MK2206. c–l n = 3 biological independent experiments
