Fig. 1
SPI1 and PERK expression was significantly decreased in osteoarthritic articular cartilage. a IHC of SPI1, PERK, and GCN2 expression in cartilage from OA patients (n = 10) and mechanical overload-induced mice (n = 6). Representative images and IHC scores are shown. Scale bars: 50 μm. b WB analysis of SPI1, PERK and GCN2 protein levels in cartilage from OA patients (n = 4). RT-qPCR was used to detect the mRNA expression of SPI1, PERK, and GCN2 in cartilage tissues of OA patients (n = 30) and control groups (n = 20). c RT-qPCR of SPI1, PERK and GCN2 mRNA expression in cartilage tissues of mechanical overload-induced model mice and control mice (n = 6 per group). d WB analysis of SPI1, PERK and GCN2 expression in chondrocytes of OA patients and control groups (n = 6 per group). e RT-qPCR of SPI1, PERK and GCN2 mRNA expression in chondrocytes of OA patients and control groups (n = 6 per group). f IF was used to observe the expression sites of SPI1 and PERK in OA chondrocytes and control groups (n = 4 per group). Scale bars: 50 μm. The experiments were independently repeated three times. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 versus the control group. ns indicates not significant. t-test
