Fig. 7
SPI1 and PERK alleviated OA cartilage degeneration in mechanical overload-induced mice. a Adeno-associated virus injection into the joints of mice for 4 weeks is schematic, and samples are harvested at 35 days. b Safranin O/Fast Green staining and OARSI scores were used to assess cartilage destruction in mouse articular cartilage. Scale bar: 200 μm. c Analysis of BMD and knee joint bone histomorphometric parameters using micro-CT data in mice. Original magnification ×50. d IHC and IHC scores were used to evaluate the expression of SPI1, PERK, P16 INK4A, P21, Col2a1, and MMP13 after the injection of AAV-SPI1/AAV-PERK. Scale bar: 50 μm. Experiments were independently repeated three times. The animal sample size is n = 4. The data were expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1 versus the Control group, #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.000 1 versus the 13.5 N + AAV-Control group. One-way ANOVA
