Fig. 2
Age-specific structural impairments in bone in prematurely aged PolgA mice and WT littermates. a Representative 3D reconstructed longitudinal micro-CT image of right femurs showing cortical and trabecular bone regions used for analysis. b 3D cortical bone images of male and female mice at 20 and 40 weeks indicating the thinning of cortical bone and increased cortical bone area with aging. c 3D trabecular bone images of male and female mice at 20 and 40 weeks, showing the reduced trabecular bone volume with age in PolgA mice. d–f Decreased cortical bone parameters with aging in PolgA mice: cortical area fraction (Ct.Ar/Tt.Ar) (d), cortical area (Ct.Ar) (e), and cortical thickness (Ct.Th) (f). g–i Altered trabecular bone parameters with age in PolgA mice: bone volume fraction (BV/TV) (g), trabecular thickness (Tb.Th) (h) and trabecular spacing (Tb.Sp) (i). n = 8–16 mice/group. Significance was determined by two-way ANOVA with Tukey’s post hoc test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1)
