Fig. 4
Degeneration of osteocyte and LCN in PolgA mice and WT littermates with age. a Representative 3D reconstructed Phalloidin-Hoechst stained images showing altered osteocytes and their dendrites with age in male and female PolgA mice 40 weeks. b Quantification of osteocyte showing the reduction in osteocyte density and (c) dendricity of male and female PolgA mice at 40 weeks. d Image processing workflow for the connectomics analysis of LCN showing, FITC stained confocal images, contrast enhancement, binarization, and skeletonization steps for osteocyte network analysis. e–g Quantification of LCN parameters showing degeneration with aging in PolgA mice at 40 weeks: lacuna density (e), canalicular length (f) and canalicular density (g). n = 3–4 mice/group. Statistical significance was determined by non-parametric mixed effect analysis with Uncorrected Fischer’s LSD test, (P* < 0.05, P** < 0.01, P***< 0.001)
