Fig. 5
Disrupted LCN connectivity with aging in PolgA mice. Changes in the nodal analysis of the osteocyte network in PolgA mice and WT littermates showing classification of nodes from the connectomics analysis (a), and the number of nodes indicating the loss of network connection with aging (b). c Mean node degree (connections per node) showing the maintenance of network architecture. d–j Proportion of tree (t)-nodes (d), proportion of cluster (c)-nodes (e), proportion of end point nodes (f), illustration of the distance of the bone matrix from lacunae and the canalicular network (g), distance to the nearest cells through the matrix (h), the average distance to LCN from the matrix (i), and distance of the network from the next lacuna (j). n = 3–4 mice/group. Statistical significance was determined by non-parametric mixed effect analysis with Uncorrected Fischer’s LSD test, (P* < 0.05, P** < 0.01, P***< 0.001)
