Fig. 2
Acceleration of osteoarthritis progression in mice by intraperitoneal injection of lopinavir. a Flowchart of animal experiment. b, c Representative images and quantitative analysis of 3D-reconstructed calcified tissues in different groups of mice at 4 weeks after DMM surgery, n = 5 or 6 per group. d Representative images of Safranine O staining, HE staining, and immunofluorescence staining for Sox9, Zmpste24, and p21 in different groups of mice 4 weeks after DMM surgery. Scale bar: 100 μm; e–i Quantitative analysis of OARSI score (e), synovial inflammation score (g), Safranine O-positive cartilage area (f), Sox9-positive cell ratio (h) and p21-positive cell ratio (i). Data are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.000 1
