Fig. 1
High-fat diet (HFD) feeding induces obesity, glucose intolerance, insulin resistance, and peripheral neuropathy. Normal diet (ND) or HFD feeding in C57BL/6J mice was instituted on week 4 of life, with analysis up to week 16 of life. a Representative Dual-energy X-ray absorptiometry (DXA) images of body fat. b Body weight after 0, 4, 8 and 12 weeks dietary treatment. n = 8 mice per group. c Fat mass and d Percentage of fat mass after 12 weeks dietary treatment. n = 8. e Fasting blood glucose after 12 weeks feeding. n = 8. f Blood glucose concentrations 0–120 min after glucose injection (1 g/kg body weight ip) in fasted mice following 12 weeks dietary treatment. n = 8. g Blood glucose concentrations 0–120 min after insulin administration (0.5 unit/kg ip) in fasted mice following 12 weeks dietary treatment. n = 8. h, i Images and quantification of Protein Gene Product 9.5 (PGP 9.5) immunofluorescent staining (green) in sagittal sections of mice hind paw skin after 12 weeks dietary treatment. n = 5. Scale bar: 100 µm. j Paw thermal withdrawal latency measurements at 0, 4, 8 and 12 weeks of dietary treatment. n = 8. Graphs represent average values ± 1 SD, *P < 0.05, **P < 0.01, and ***P < 0.001. Comparisons between groups were analyzed by unpaired Student’s t test. In subfigures (b and j), comparisons between groups were analyzed versus D7
