Fig. 6

DRG neural conditioned media effects on periosteal cells after dietary intervention. a Schematic representation of mouse DRG neuron-derived conditioned medium (CM) and treatment of mouse periosteal cells derived from ND or HFD dietary treatment for 12 weeks. b Cell proliferation assay of periosteum mesenchymal cells isolated from ND or HFD-fed animals after 24, 48 and 72 h treated with DRG neuron-derived CM or control media. c Gene expression for osteogenic markers Runt-related transcription factor 2 (Runx2) and Alkaline Phosphatase (Alpl) after 72 h treatment with or without CM. d Quantification and staining for alizarin red 2 weeks after osteogenic differentiation with or without CM treatment. e PCA analysis of total protein extracted 2 wks after osteogenic differentiation with or without CM treatment. f Quantification of individual proteins from MAPK protein array. n = 3 per each group in the in vitro experiment. Graphs represent average values ± 1 SD. Two-tailed ANOVA with post-hoc analysis was performed between the four experimental groups. *P < 0.05, **P < 0.01 and ***P < 0.001