Fig. 2 | Bone Research

Fig. 2

From: Inflammatory macrophage-derived itaconate inhibits DNA demethylase TET2 to prevent excessive osteoclast activation in rheumatoid arthritis

Fig. 2

Genetic ablation of ITA-producing enzyme Irg1 induces a more severe bone destruction phenotype in TNF-Tg-induced RA mouse model. a Arthritis scores of Irg1+/+, Irg1−/−, Irg1+/+/TNF-Tg, and Irg1−/−/TNF-Tg mice from 6 to 12 weeks of age (n = 6). b Representative photographs of rear paws of Irg1+/+, Irg1−/−, Irg1+/+/TNF-Tg, and Irg1−/−/TNF-Tg mice (n = 6). Scale bar: 1 cm. c Representative μCT reconstruction images of the rear paws and talus of four groups (n = 6). Scale bar: 1 mm (top), 50 μm (bottom). d, e Erosion scores of the rear paws (d) and measurement of BV/TV of talus (e) of four groups (n = 6). f Representative images of HE staining and TRAP staining of ankle joints of Irg1+/+, Irg1−/−, Irg1+/+/TNF-Tg, and Irg1−/−/TNF-Tg mice (n = 6). Scale bar: 200 μm (top), 100 μm (bottom). g Synovitis scores based on HE staining of ankle joints (n = 6). h Quantification of osteoclast numbers around the ankle joint based on TRAP staining (n = 6). Significant differences were determined by Mann-Whitney U-test (d, g) and Student’s t-test (e, h). Data represent means ± SD for each group

Back to article page