Fig. 2 | Bone Research

Fig. 2

From: The mitochondrial E3 ligase MAPL SUMOylates Drp1 to facilitate mitochondrial fission in intervertebral disc degeneration

Fig. 2

MAPL SUMOylates Drp1 and facilitates its recruitment to mitochondria. a The levels of total MAPL, cleaved caspase-3, Bax, Bcl-2 and SUMO1 in TNF-α-stimulated NPCs transfected with the MAPL overexpression plasmid were analysed by Western blotting. b, c Western blot analysis of the mitochondrial and cytosolic levels of Drp1, MAPL, and SUMO1 in NPCs after MAPL overexpression with or without TNF-α treatment. d SUMO1 modification of Drp1 was analysed by co-IP in MAPL-overexpressing NPCs under inflammatory conditions. e Mitochondrial fragmentation caused by MAPL overexpression was observed via MitoTracker Green staining. f The colocalization of Drp1 and MitoTracker Red was observed via confocal microscopy. (n = 3; *P < 0.05, **P < 0.01, and ***P < 0.001)

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