Fig. 5
SENP5 negatively modulates Drp1 SUMOylation and mitochondrial fission. a Western blots showing the levels of total SENP5, cleaved caspase-3 and SUMO1 in NPCs after transfection with the SENP5 overexpression plasmid under inflammatory conditions. b, c Drp1 and SUMO1 expression in the cytosolic and mitochondrial fractions of the TNF-α-treated NPCs after SENP5 overexpression was measured via Western blotting. d Co-IP analysis of the SENP5-induced SUMO1 modification of Drp1 after TNF-α treatment. e Western blot analysis of total SENP5, cleaved caspase-3 and SUMO1 levels in NPCs after SENP5 knockdown and TNF-α treatment. f, g Mitochondrial and cytosolic levels of Drp1 and SUMO1 in TNF-α-treated NPCs transfected with si-SENP5 were assessed by Western blotting. h Drp1 SUMOylation after SENP5 silencing was analysed via co-IP. (n = 3; *P < 0.05, **P < 0.01, and ***P < 0.001)
