Fig. 6 | Bone Research

Fig. 6

From: The mitochondrial E3 ligase MAPL SUMOylates Drp1 to facilitate mitochondrial fission in intervertebral disc degeneration

Fig. 6

SUMOylation and mitochondrial translocation of Drp1 are coregulated by MAPL and SENP5. a Western blots showing the levels of total MAPL, SENP5, cleaved caspase-3, and SUMO1 in NPCs transfected with OE-MAPL or OE-SENP5 plasmids. b, c Mitochondrial and cytosolic levels of Drp1 and SUMO1 in MAPL-overexpressing and SENP5-overexpressing NPCs were assessed using Western blotting. d Co-IP of SUMOylated Drp1 in NPCs overexpressing MAPL and SENP. e MitoTracker Red staining and immunofluorescence staining for Drp1 in NPCs after MAPL or SENP5 overexpression. f Changes in the morphology of mitochondria in NPCs transfected with OE-MAPL or OE-SENP5, as shown by MitoTracker Green staining. (n = 3; *P < 0.05, **P < 0.01, and ***P < 0.001)

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