Fig. 3

Plasma from bone defect diabetic mice promotes epidermal cell proliferation and migration by enhancing fibroblasts to secrete KGF. a Quantitative statistics of the percentage of Edu⁺ L929 cells by flow cytometry. Cells were treated with 10% FBS (Blank) and 10% FBS combining 5% control diabetic mice plasma (Control) or bone defect diabetic mice plasma (Bone defect), respectively (n = 4) for 48 h. b The average migration distance of L929 cells in different groups was quantitatively analyzed according to scratch test (n = 8). Yellow dashed line marks scratch wound edges. c Relative mRNA expressions of L929 cells by qPCR (n = 3). Cells were treated with 10% FBS (Blank) and 10% FBS combining 5% control diabetic mice plasma (Control) or bone defect diabetic mice plasma (Bone defect), respectively for 72 h (n = 4). d Schematic illustration of the L929 cell conditional medium harvesting and culture of HaCaT cells. e Relative protein expressions of L929 cell conditional medium by Elisa (n = 5). f Western blot analysis and the relative level of phosphorylated FGFR-2, Erk1/2, and P38 in HaCaT cells after treating with the indicated CM for 20 min (n = 3). g Quantitative statistics of the percentage of Edu⁺ HaCaT cells by flow cytometry. Cells were treated with L929-CM, L929-Control-CM, and L929-BD-CM, respectively for 48 h (n = 4). h The average migration distance of HaCaT cells treated with differenced CM was quantitatively analyzed according to scratch test. The yellow dashed line marks scratch wound edges (n = 8). Scale bar, 200 μm. i KGF protein concentrations by ELISA in wound tissue on days 3 and 7 (n = 4). Data are presented as mean ± SD and statistical significance was analyzed via one-way ANOVA with Tukey’s multiple comparison test for (a, c, d, f, g), two-way ANOVA with Tukey’s multiple comparison test for (b, h, i). P value: *P < 0.05, **P < 0.01, ***P < 0.001