Fig. 6

Bone derived factors from bone defect diabetic mice efficiently promote diabetic wound healing. a Representative images of dorsal wound treated with Tegaderm dressing (Control), Collagen hydrogel (Collagen), Collagen hydrogel containing bone-derived factors (Coll-BDFs), and Collagen hydrogel containing bone-derived factors of diabetic bone defect mice (Coll-BD/BDFs) at different times. Scale bar, 5 mm. b Quantification of the percent of dorsal wound residual area at indicated times in different groups (n = 6). c Sagittal sections of dorsal wound tissue immunolabeled for cytokeratin (red) and integrin a5 (green), cell nucleus for DAPI (blue) at days 3 and 7. Scale bar, 200 μm. d Representative H&E staining of dorsal wound tissue on days 7 and 14. Scale bar, 200 μm. e Sagittal sections of day 14 wounds tissue immunolabeled for CD31 (red) and cell nucleus for DAPI (blue). Scale bar, 200 μm. f Representative Sirius red staining of dorsal wound tissue on day 14. Scale bar, 200 μm. Data are presented as mean ± SD and statistical significance was analyzed via two-way ANOVA with Tukey’s multiple comparison test for (b). P value: *P < 0.05, **P < 0.01