Fig. 6

cPLA2 inhibition protects against OA in a surgically induced model. a Schematic of the experimental outline. 12-week-old male WT mice underwent DMM or sham surgery. Starting at 4 weeks post-surgery, the mice were treated daily with either 10 mg/kg body weight FFD or 10 mg/kg body weight AACOCF3 for a duration of eight weeks (n = 6). b Representative Safranin O/Fast Green stained images of knee joints treated with/without FFD or AACOCF3 at 12 weeks post-surgery (n = 6). Scale bar = 100 μm. c, d 2 min travel distance (c) and von Frey pain assay (d) in mice with varying treatment at the indicated time points after surgery (n = 6). e Representative immunohistochemical staining images of p-cPLA2, cPLA2, Col2, Mmp13, aggrecan neoepitope, p16, and p21 in knee joint sections from each group (n = 6). Scale bar = 50 μm. f Quantification of positive staining based on IHC images in (e) (n = 6). c, d, f Data are mean±s.d., P-values by one way ANOVA with Bonferroni post hoc test. *, P < 0.05, **, P < 0.001, ***P < 0.001