Fig. 3
From: WSB-1 regulates the metastatic potential of hormone receptor negative breast cancer
WSB-1 affects the expression of pro-tumourigenic and pro-metastatic factors in a HR-dependent manner MCF7 and MDA-MB-231 cells were transfected with WSB-1 (siWSB-1) or non-targeting siRNA (siNT). Cells were exposed to 20% or 2% O2. a–c WSB-1 and HIF1α protein levels were determined by Western blot. Representative blots (n = 3) are shown in a, densitometry in b and c. d–g Transcript levels of WSB1 and other HIF-dependent genes were assessed after 24 h exposure to 20% or 2% O2. Histograms represent average of n = 3 experiments. *p < 0.05; **p < 0.01; ***p  <  0.001;****p < 0.0001
